Screening and Confirmation of Anabolic Steroids Using Accurate Mass LC/MS Application
نویسندگان
چکیده
The use of the Agilent LC/MSD TOF, an orthogonal-axis time-of-flight (oa-TOF) mass spectrometer, equipped with an atmospheric pressure chemical ionization (APCI) source, is described for the analysis of four anabolic steroids and one internal standard, in urine extracts at a 1–2 ng/mL concentration. The high degree of mass accuracy (<3 ppm) of the TOF instrument allows for both excellent confirmation by empirical formula determination and quantitation using extracted ion chromatograms (EICs). The accurate mass capability of the LC/MSD TOF produces EICs with sufficiently narrow mass widths to exclude most chemical noise contributions. For increased chromatographic resolution and speed, a C18 column with a 1.8-micron particle size was employed. Screening and Confirmation of Anabolic Steroids Using Accurate Mass LC/MS Application
منابع مشابه
June 2012
nabolic androgenic steroids (AAS) and other anabolic agents are widely abused as performance-enhancing drugs by athletes of all abilities—from elite competitors to participants in amateur sports to high-school students. AAS and other anabolic compounds are banned by most amateur sports anti-doping programs, the World Anti-Doping Agency (WADA), and many professional sports organizations (1). In ...
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